FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50586621
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG593120.11724839261353313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG200650.015.27234335
AGCTTAG201450.015.23440645
ATAGGGG207950.015.02850338
GGGAGGT297000.014.1849113
GGAGGTC290900.013.9712674
GAGCTTA225850.013.61842344
TGGCATA228200.013.43841234
CATAGGG235450.013.02475637
GTCACTC297050.012.9900138
TCAGACG94150.012.9077532
GGTCACT305600.012.7885557
GCATAGG244150.012.57895336
ACTCTCC312500.012.47759311
GTTGTCG24600.011.70657126
TGGGAGG293400.011.3138172
GAGGTCA379000.011.0961475
CGTGGCG162600.010.924491
AGGTCAC380400.010.8239776
TAGGGGA366600.010.76504939
TCACTCT371800.010.5903669
Produced by FastQC (version 0.11.5)