FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88737097
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2272840.2561318858560361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT412600.026.79343245
AGACCGC455700.024.3882644
GACCGCG476250.023.1983645
ACCGCGT479400.023.0743526
CAGACCG486300.022.964843
AGAACGA483950.022.88512444
CCGCGTT501600.022.0393777
CGCGTTC506700.021.835428
AATACGG530450.020.85126732
ATACGGA529800.020.8216233
TACGGAG529600.020.77423334
TCAGACC570550.020.0035952
AAGAACG566350.019.5396243
GCGTTCT568600.019.517689
CAATACG572500.019.37481131
ACGGAGA590150.018.7639535
CCCAATA598750.018.67576229
CTCAGAC681350.018.2316421
CCAATAC639850.017.67313830
CACTCCC662250.017.12484724
Produced by FastQC (version 0.11.5)