FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88737097
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG220250.015.91595945
GGCATAG237600.014.71585135
CATAGGG260650.013.48351537
ATAGGGG263050.013.40327738
GAGCTTA279350.012.84675144
ACCCGGC173150.012.7216945
GCATAGG278150.012.61094536
GTCACTC385500.012.577878
ATAGCTC165450.012.38887639
TCACCCG190400.011.9236423
GGTCACT406200.011.9202837
TGGCATA305150.011.69420234
ACTCTCC435350.011.1531211
ACGGACA210450.011.044209518
CGGACAG194450.011.015697519
TAGCTCT186650.010.99379540
GGGAGGT504500.010.9044163
AGGTCAC470650.010.8759726
ACACGGA204850.010.72006316
TAGCGTA14600.010.47938737
Produced by FastQC (version 0.11.5)