FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49086471
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT592490.1207033196580785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG35600.025.019756
ACCGCGT134750.022.5676176
GACCGCG135100.022.4758575
AGACCGC139550.021.920324
TCGACGA41800.021.6315467
CCGCGTT147900.020.6067247
CGCGTTC149500.020.356098
CGACGAG43400.020.2639858
CGGTATA55200.020.21065720
CAGACCG160800.019.3312453
GTTCGAC47800.018.4927925
GAACGAT176400.017.58869645
TCAGACC191050.017.0474472
CTCAGAC251450.015.6061261
TACGGAG197150.015.367834
AATACGG199850.015.34025732
ATACGGA201100.015.30082833
GCGTTCT202750.015.1651089
AGAACGA214950.014.58223344
CCCGTAT31800.014.5388611
Produced by FastQC (version 0.11.5)