FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50845873
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT891790.17539083260503757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG220950.022.0527215
AGACCGC224800.021.895154
ACCGCGT226150.021.7326586
TTCGACG27600.020.9450826
CCGCGTT240050.020.4740777
CAGACCG243850.020.3875923
CGCGTTC244550.020.115358
TCAGACC287950.017.9214342
GCGTTCT275650.017.8946889
GAACGAT276050.017.75144245
GTTCGAC32750.017.5156655
CTCAGAC354500.016.3845421
CGGTATA47200.015.58590920
TCGACGA38250.015.1720017
AGAACGA327900.014.98575344
AATACGG323650.014.93041132
GTCCGTT38850.014.8811471
ATACGGA325950.014.88028733
AACGCTA188050.014.85815123
ACGCTAC188300.014.84008924
Produced by FastQC (version 0.11.5)