FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50845873
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG763990.1502560492962723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGC86250.017.868216
GTTGTCG85800.016.88641726
TATAGCT102600.015.34983357
GCTAAGT98200.015.307590514
GTCGTTC96600.014.9981729
TGTCGTT98500.014.86874428
GGAGGTC417300.014.8539094
GGGAGGT428450.014.8244393
GAGGTCA440150.014.1492345
CGTTCCC104100.013.72302831
AATATAG126750.013.5794135
CAATATA123100.013.4885684
TCGTTCC106900.013.3636530
AGGTCAC476850.012.8709966
AGCTTAG332950.012.83553145
GGTCACT481000.012.5589177
TACAATA134550.012.4075252
CTAAGTG124600.012.37127315
TGGGAGG369250.012.332612
GTCACTC489450.012.274628
Produced by FastQC (version 0.11.5)