FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48254181
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA534990.1108691493489445No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG523120.10840925887852082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG153050.015.13787945
GGCATAG162150.014.2160435
ATAGGGG169700.013.87573938
GAGCTTA179350.013.04435244
GTCACTC228350.012.8508568
CATAGGG183900.012.70651337
TGGCATA186600.012.40085234
GCATAGG190850.012.1848936
GGTCACT243450.011.9524337
GGGAGGT277950.011.7410953
ACTCTCC253100.011.5053411
TCAGACG99250.011.4054372
TAGCGTA7550.011.35232937
GGAGGTC271750.011.2896264
AGGTCAC281900.010.9437296
CCGTACG23400.010.5578444
GAGGTCA300550.010.4242565
AACTCTA86300.010.19487345
GTTGTCG24850.09.9584526
TCACTCT299350.09.9227749
Produced by FastQC (version 0.11.5)