FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55625543
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAC144650.020.2995524
AACGCTA144850.020.17826523
CGCTACC153550.019.06480225
ACCGCGT98750.018.02076
AGACCGC102550.017.268034
TCACGCT180750.016.964848
AAAACGC174550.016.88560121
GTCCGTT44650.016.6636311
ACGTTCG27200.016.6249146
GACCGCG107350.016.4950085
ACGCTCA208950.016.43015510
CCGCGTT109450.016.3000777
CGCGTTC111900.016.003518
GAAAACG186600.015.87958520
CAGACCG119450.015.3335233
CCCGTAT37250.015.131811
TGCGCGT39450.014.88486345
TTCACGC210600.014.8380257
TCGGCGC81500.014.82409145
TACGGTA85800.014.6579644
Produced by FastQC (version 0.11.5)