FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50723846
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT897630.1769641048117684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG31500.025.0630746
GACCGCG179500.024.8733255
ACCGCGT182450.024.44656
AGACCGC187950.023.8866924
CCGCGTT193700.023.1660027
CGCGTTC196850.022.8638578
GAACGAT193100.022.86026645
CAGACCG197950.022.8390673
CGGTATA51050.021.14868220
TCGACGA38950.020.7889637
AATACGG229900.019.34235632
TCAGACC239450.019.3034442
ATACGGA231150.019.24748833
TACGGAG231000.019.1139334
AGAACGA235150.018.8601744
CGACGAG42250.018.686088
CAATACG244900.018.39644231
GCGTTCT253400.017.8502259
AACGCTA158450.017.80094723
AAGAACG250100.017.75977343
Produced by FastQC (version 0.11.5)