FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50723846
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG144000.014.11302638
GGCATAG144700.013.62177535
AGCTTAG145700.013.61920945
CATAGGG155950.012.72852637
GTCACTC211200.012.6994088
GTTGTCG29100.012.13701426
GGTCACT220300.011.9602937
GGGAGGT237200.011.9061833
GCATAGG165700.011.88448736
GAGCTTA169400.011.80757644
GGAGGTC240550.011.4029224
ACTCTCC242500.011.27378911
AGGTCAC248600.011.0965266
TGGCATA182400.011.06437234
GAGGTCA270550.010.3712215
TAGGGGA254100.010.30963339
TCACTCT278550.09.7743059
GCGTAGG64550.09.48347636
CACTCTC279850.09.45552510
CGTAGGG65300.09.3400937
Produced by FastQC (version 0.11.5)