FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48430880
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG43000.026.4739366
CGACGAG48850.022.9811848
TCGACGA50650.022.5642417
GTTCGAC52400.021.8965325
CGGTATA67400.021.66373620
ACCGCGT54800.018.1458856
GACCGCG55000.018.0798995
ACGAGGA79000.017.54253410
AGACCGC57200.017.5418454
ATGACGG81350.017.09175316
CAGTTCG76150.016.574083
AGTTCGA73750.015.954244
GACGAGG86900.015.6629289
CCGCGTT64050.015.490167
GTTCCTA99850.015.43470115
GTCCGTT43200.015.3279031
AACGCTA137800.015.29941423
ACGCTAC139000.015.1680724
CGCGTTC66800.014.8524678
CCCGTAT40700.014.83062651
Produced by FastQC (version 0.11.5)