FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48430880
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG35100.016.53894626
TGTCGTT44700.013.03528828
ATAGGGG161350.012.98049438
GGCATAG165350.012.29926635
AGCTTAG167700.011.97873545
ATATAGC61700.011.4878326
CATAGGG177000.011.464237
GGGAGGT233550.011.282063
GCATAGG181650.011.24507136
TCGTTCC51200.011.16086930
GGAGGTC228500.011.0488854
GCTAAGT54050.010.86652614
GTCACTC223150.010.7289338
GGTCACT225750.010.6551927
GAGCTTA192650.010.42737444
TATAGCT66750.010.4164227
AGGTCAC242750.010.3261556
TGGCATA199250.010.31980134
TTGTCGT60600.010.13596327
GAGGTCA251850.010.0334355
Produced by FastQC (version 0.11.5)