FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52385898
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1132160.21611923117171727No Hit
CCCCACTTTGCAAACTGGATGCAGCATAGATCAGGAGCTTAGGGGCTTTCC753090.1437581541505693No Hit
CTGGGCTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTG655250.12508137208987044No Hit
CTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGG579850.11068818558765568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCTT302950.021.3166482
TCACGCT285950.021.1185028
TTCACGC290050.020.8355547
GCCGGCT318700.020.392271
CGGCTTC337050.019.5739143
TTCGACG17300.019.2481236
ACGCTCA317000.019.24155410
AACGCTA312450.018.85986323
ACGCTAC312900.018.80397424
CACGCTC323200.018.6287889
CGCTACC318950.018.41907525
AAAACGC340750.017.3925921
GAAAACG349850.016.88264720
AAACGCT355950.016.68148822
GTCCGTT33350.016.5375461
CTACCTC381950.015.71637927
CGTGGTT379200.015.64057741
GTTTTCG385650.015.37302145
GCTACCT388300.015.33772626
CCCGTAT27050.015.2294651
Produced by FastQC (version 0.11.5)