FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52385898
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA579370.11059655787517472No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG560170.10693144937593702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG188850.015.10792545
ATAGGGG206950.014.05775938
GGCATAG208550.013.68041135
GGGAGGT285050.013.5712923
GGAGGTC276850.013.5155734
GAGCTTA220250.012.99492444
GTTGTCG24450.012.69943826
TGGCATA237900.012.15343934
GTCAGGA201800.012.0691621
CATAGGG243000.011.84234637
CACCGAA255000.011.4534745
GCATAGG264950.010.92095136
TGGGAGG289950.010.708142
GCACCGA274550.010.58872444
GTCACTC363450.010.1856048
TGTCGTT32200.010.13225228
GGTCACT361650.010.0868727
AGGGATC136550.010.043422
TAGGGGA362450.09.96351239
TCAGACG111200.09.3811642
Produced by FastQC (version 0.11.5)