FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004852265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290417151
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACGCC12152550541.84515431734953No Hit
CTAGGTGA8397180028.914201420562797RNA PCR Primer, Index 38 (100% over 8bp)
AGCCAATT7932665227.314727014865593No Hit
TCGTTCAG25135280.8654888292048564No Hit
GATACGCA6174470.21260693381018672No Hit
AGCCAATA4581060.15774068384824835No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)