FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004852299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109588561
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGCCTA3365030930.706041481829477No Hit
GCTCAACC2866154726.153776213924374No Hit
CAAAGTGG2431175422.184572712839984ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
TTGTTGAT1999723318.24755505275774No Hit
GTGTTGAT10184690.9293570338969959No Hit
CGCGCCTA1593730.14542849960407822No Hit
CAAAGTGA1345800.1228047879924256No Hit
GCTCAACA1335190.12183662125100812No Hit
AGCGCTTA1270270.11591264529880996No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)