FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32464625
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA140450.018.1468071
GACCAAT156350.017.9101851
TGGCTGG193600.017.8714561
TGCGTGG160650.017.8143731
GAACCTA170950.017.7160631
CCGGGAT89700.017.6023731
GGGACCT104500.017.5551381
CTACCCA116150.017.5337981
CGTTGGG207900.017.5314061
TTGGCGC130700.017.51965510
AGCTGAA114500.017.493168
TACGGAT122850.017.4820671
CTGATAG138050.017.4670961
GGGAGAT103050.017.4492471
CGGAGTC150900.017.4256741
CTGAAGT142250.017.398771
GGCGACT85250.017.3954771
ATCTACT225750.017.3399831
CAGGAAT148700.017.2607678
GGCTCGA99800.017.1874641
Produced by FastQC (version 0.11.5)