FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004852463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74072245
Sequences flagged as poor quality0
Sequence length91
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21745662.935736590675765Clontech SMART CDS Primer II A (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3148780.42509579667795944No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3002030.40528405747658924Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1382300.18661510799355413Clontech SMART CDS Primer II A (100% over 25bp)
GGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATC950790.1283598195248436No Hit
GCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGG906890.12243317318112877No Hit
GTCCAAGGGTAGACCACCAGCAGCCTGCCCAGGGCCTCACCACCAACTTC888450.1199437117101014No Hit
GGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGT844550.11401706536638657No Hit
GTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCC844520.11401301526637947No Hit
GGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGTAGACCA839150.11328804736510957No Hit
GATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGTAGACCACCAGC826500.11158025519545142No Hit
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAA816040.11016812032631117No Hit
CAAAGAACCTCTGGGTCCAAGGGTAGACCACCAGCAGCCTGCCCAGGGCC805580.10875598545717091No Hit
CCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTC762880.10299134311373982No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)