FastQCFastQC Report
Tue 28 Jun 2022
EGAF00004852464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77576645
Sequences flagged as poor quality0
Sequence length91
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16585172.137907613818566Clontech SMART CDS Primer II A (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2661030.3430194744823007No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2386570.30764026982605397Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1166400.15035453002640164Clontech SMART CDS Primer II A (100% over 25bp)
GCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCAC1150040.14824564790085984No Hit
GTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGA1037820.1337799540054871No Hit
GTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCC1022310.13178064093903522No Hit
GGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGTAGACCA979510.126263516551921No Hit
GGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGT948780.12230227280388317No Hit
CTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCATCAGGA894010.11524215825523261No Hit
GCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACC881490.11362827046722632No Hit
GTCCAAGGGTAGACCACCAGCAGCCTGCCCAGGGCCTCACCACCAACTTC838500.10808665417278615No Hit
GATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGTAGACCACCAGC834860.10761744079033064No Hit
CAAAGAACCTCTGGGTCCAAGGGTAGACCACCAGCAGCCTGCCCAGGGCC826830.10658233544386973No Hit
CCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACCT813810.1049039952681635No Hit
CTCAAAGAACCTCTGGGTCCAAGGGTAGACCACCAGCAGCCTGCCCAGGG790700.10192500590867264No Hit
GCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGG790470.10189535781033067No Hit
GGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATC781970.10079966721943184No Hit
CCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTC777680.10024666573296641No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)