FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19020991
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGC119550.0114.14031
TACACGA66200.0110.103331
CTAATAG139900.0109.747793
TTGCCGT131750.0107.526591
CTGATAG136150.0106.154211
TATTACC121900.0105.858161
CGGTTAA103000.0103.618281
CCTTACG60950.0102.9678341
CGTATAG58400.0102.330953
GCGCGAT76700.0100.485441
CACCGTA79450.099.458539
GCCGTAG91450.097.467713
TCTAACG66900.097.329559
CGTAACA77650.096.950163
ACACCCT173800.096.630771
GATTACG29500.096.621932
GACCAAT117850.096.354041
TACCTTA106300.096.1893541
TTCGAAG144900.096.147281
CGGACGT78600.096.0018841
Produced by FastQC (version 0.11.5)