FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19027887
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAG639750.0115.59751145
TAAACCG103000.0115.2959061
CGGACGT114150.0113.421461
GCATGCG96550.0109.7709661
ATCGACG47800.0104.249019
CCACCTA163900.0102.503671
GTCCGAA77400.099.753388
CGTACGT52850.098.667013
CTAGAGT156650.097.549031
TACGGGC79700.097.0632551
CCTTACG68450.096.824861
CGTAGGC89900.096.74561
CGTCCGA58750.096.530667
CTCGTAA52000.095.6896065
AACGTTG80750.095.528451
ACGGGTC82550.095.490411
TCGTTAC60300.094.5298168
CGTTGAG62150.094.40513
CCATAAC137400.094.146927
TCGACTA33450.093.3238149
Produced by FastQC (version 0.11.5)