FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18678068
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG243560.13039892562763986No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCC238000.0128.148359
CAACCGC241350.0126.7600568
ACAACCG241650.0126.452847
CTCGACA126200.0125.797043
AAGCGTA168750.0125.7130369
GCTCGAC130750.0122.811382
TAGCGAG149300.0122.703683
CAAGCGT177650.0122.676928
TCGACAT126850.0121.992534
GGCTCGA179350.0121.606231
CGTCTAC198050.0121.53671
CGTAGCG203450.0118.743681
CGACATG134500.0118.630965
AGGCGTA107350.0114.8321158
TCGTAGG108750.0114.086428
CTCTACG166400.0113.120791
GATCGCG146700.0113.104231
CCAAGCG194850.0112.888647
TACACCG90000.0112.745789
CAGCCTA211400.0112.170748
Produced by FastQC (version 0.11.5)