FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13939755
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC196090.1406696172206757No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA188450.13518888961821782No Hit
CGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGG184060.13203962336497305No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGT182690.13105682273468938No Hit
CACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTT165590.11878974917421431No Hit
CAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGC157670.11310815720936272No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC150970.10830175996636955No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAG428200.0108.69201145
CGATTGA45850.0103.905781
ACTAACG34500.097.9656452
TACGGTA44250.095.9746551
TGCGATA45050.095.038799
ATCCGAA67450.094.391041
CCGATCT107900.094.265748
CTCGTAC69950.092.475471
ACCGTAA59700.090.293951
GCCTCTA179750.090.2105941
GTCCGAA59600.090.191558
TACGGGC47100.088.4661
CGTAACA41900.088.442143
TATCCGT56700.087.611253
CGAACAT54200.087.360541
CGTCATA51550.086.568577
TTATCCG59550.084.403112
CCTTACG39750.084.298911
TAACTCG48500.082.8466642
GCATACA118400.082.812261
Produced by FastQC (version 0.11.5)