FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15189614
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA53650.0117.0828551
TTACGGA52250.0112.014079
CGCCATA64500.0111.179069
TAAACCG44400.0108.814391
AGCGTAA49350.0107.8816769
TGCTACC121950.0105.7065351
TACCTTA84200.0104.1140441
CAATGGA115600.0104.0154348
CGATCGG67300.0103.513881
ATCGTGC46050.0102.556045
GTACTCG70600.0102.085948
TATGCCC88200.0100.3011551
TAGGCAT127500.0100.190731
AGTGTCA174000.0100.093171
GGTTACG48750.099.6987467
CTAACGG36850.099.659349
CGCTATC64000.099.400851
GATCGTA56800.099.2998051
CGGCTAG74400.099.218431
TCCCGAT73650.098.9425351
Produced by FastQC (version 0.11.5)