FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004852486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15458747
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG1568501.0146359210096394No Hit
CAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGC719190.4652317551998231No Hit
CAGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA373650.24170781758702692No Hit
AAACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC316630.20482255127145815No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC268280.17354576020941412No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA212640.13755319237710534No Hit
TAGGATAGACTAGATGCAATTCCACTTTCTTATATGGGGGCACTTGAACA205290.13279860262930754No Hit
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGGATAGTAGTTCGCTTTG194330.1257087653999383No Hit
GGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTC188540.12196331306799962No Hit
TTCTTAGCAAGCCTATCGCTATGACCTTTCTTATATGGGGAGTCATCTCA180260.11660712216843966No Hit
TTGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACC168910.10926500058510563No Hit
TTCTCCTAGCAGATCGTGATCTCAAGTTTCTTATATGGGGACAGCTACGG162950.10540957815015667No Hit
AACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAG161650.1045686303036074No Hit
GTTCGGGAGTCAATAGCTTGCATGGCTTTCTTATATGGGGGCTTTGGAGC156120.10099136754097857No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)