FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16728767
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG313910.1876468241801682No Hit
CAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGC272290.1627675249466981No Hit
CAGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA176430.10546503516965715No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAG604850.0109.9112145
TACGGTA57600.0107.755191
CGTTGGG63150.0102.437921
TACGGAT66200.0101.5698551
TCGCGAG57250.0100.015691
CGGACTG74200.098.0805051
ATCGAGT80600.097.52311
CGCTATC57750.097.3837361
CACGCGA25700.096.993248
AACGTTG71700.096.0138241
CATCGAA78950.094.947681
CGGTTAA98550.094.765991
TAGCGCA50900.094.709913
TTAGGCA86900.093.891
CGTAGCG74450.093.83721
GCGCAAC45200.093.639441
AACGCAC63350.092.6519559
TCGATCC51000.092.3547748
AGTCGAA63050.091.8285757
GTACGAA41750.091.815378
Produced by FastQC (version 0.11.5)