FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15671568
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA285670.1822855249710814No Hit
CGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGG253390.16168771369910145No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG109300.0109.81651
GATATAG383150.0107.921776145
TCGCATG87400.0106.958528
GACTAAC146550.0106.703031
CTAGACT106450.0103.5429846
CGACTAG45200.0100.063863
CGATTGA51400.099.614551
CGACTCA75150.099.213133
CGTTATG61950.099.078467
GACTGTA148950.099.00599
CTTACCG86100.098.803031
CTAACCA139000.098.7653053
CCGTAAG54750.097.945566
GTCGACA77100.097.937837
TAGACTG114450.095.735537
ACTAACC145300.094.88272
TACACCG90300.093.732949
CCGTACT102850.093.546831
TTACCGT51900.093.440852
GCGTTAT70000.093.171196
Produced by FastQC (version 0.11.5)