FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004852491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8041300
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA313910.3903722034994342No Hit
TGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC190470.23686468605822442No Hit
TTGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT174690.21724099337171848No Hit
TTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGC167750.20861054804571402No Hit
CGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGG162060.20153457774240482No Hit
CCACCTACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG130420.16218770596794052No Hit
ACCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACT113030.140561849452203No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGT106460.13239152873291632No Hit
ACGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACC103880.12918309228607316No Hit
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG91080.11326526805367293No Hit
TACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGA81900.10184920348699836No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)