FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8678345
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGG125890.14506222096494206No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA119580.13779124936839915No Hit
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG118140.13613194681704865No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA23400.0123.134131
CGCACGA18900.0120.754836
GTCGAAC47950.0117.556538
GGCTCGA40950.0113.04411
TGCGTGG54900.0111.627131
CCGATCA36050.0111.284973
GATATAG199700.0110.44088145
TACACGA26300.0110.1127241
TACGTAG29600.0108.9241265
TCGAACA51850.0108.862189
CGATCAC30100.0107.947584
TACGGGC33850.0107.589221
AGTCGAA34850.0106.652347
ACCGAGT22450.0104.994964
TAGCGAG23950.0104.806263
CCGTCGA26150.0104.7311556
CGCTATC28950.0103.569861
ATGCGAT32850.0103.2953341
TGAGTCG35800.0103.012655
CGTCGAA30600.0102.767
Produced by FastQC (version 0.11.5)