FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14607369
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGG530110.36290587305626354No Hit
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG453370.3103707450670959No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA445260.3048187527815584No Hit
CAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGC239270.1638008870728192No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC231610.1585569584776013No Hit
TGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC223620.1530871165094823No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGT220430.15090328723810564No Hit
ACGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACC214860.1470901433379276No Hit
ACCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACT211230.14460509623601622No Hit
CCACCTACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG206970.14168875996765742No Hit
TACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGA171360.11731065327370042No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAG616950.0116.628746145
TACGGGC82950.0110.615821
CGTTCTG154300.0106.708181
CCTTACG42000.0100.043661
CGATTGA28400.098.207241
TCGCGCA38750.097.1079563
TACACGA40500.096.017261
ACGCCAG124350.095.046431
CCACCTA100450.091.707251
GATCGCG62000.090.3228761
ATCGCGC42900.089.749212
ACCGTAA56500.089.706491
TGACTAC75000.088.002219
CTCTACG65600.086.809251
ATCCGAA45100.086.708331
GTGACTA76700.085.8627858
AAACGGG72250.085.0682751
CGCTATC38450.083.901651
AACGTTG50750.082.938251
ATCGTCG21300.081.973589
Produced by FastQC (version 0.11.5)