FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2353953
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAGTAGAAGGTGATTACCAGGAATTTCTTATATGGGGAGACACATCT31140.13228811280429134No Hit
ACTTGTTAGAGAACAGGGGCCTCAGATTTCTTATATGGGGATTCGCCAAG30720.13050388006897334No Hit
AGGCCACGTAGATTAGAATTTGATGCTTTCTTATATGGGGGCTGCTTAAA29340.12464140108149992No Hit
CTAGAGTGTGTGTGCCTGCAGAATGCTTTCTTATATGGGGGTGACAATTA28530.1212003808062438No Hit
CAGTAACTCTACCTGCCGACTAGCTTTTTCTTATATGGGGTATAAACAAT27440.11656987204077567No Hit
GACTAACAGGGCTTCCCTGTTCAGTATTTCTTATATGGGGGTCAGAGCTG27410.11644242684539581No Hit
AGGGTGATCATAACCGGTATTTGATGTTTCTTATATGGGGGAAACCCAGT26480.11249162578862025No Hit
CCTAAAGTCTGCTGTCTCCCGTTCGGTTTCTTATATGGGGACTTGATTAT26240.11147206422558138No Hit
TACTCGCTCGCAGGCTTCCTGTCCGCTTTCTTATATGGGGGCATTTTGGC25910.11007016707640298No Hit
CTCGTCAAGGGATACCACTACGGCGCTTTCTTATATGGGGAGCCCAGAGA25770.10947542283129698No Hit
TATTACCTCCGTTGTCTCGCTGTAAATTTCTTATATGGGGCTTTTGGTCC25610.10879571512260439No Hit
TTGGAACCATGCATGTGAGGGCCAACTTTCTTATATGGGACTGAAGGTCA24900.10577951216528114No Hit
AAGCCGCGTGAAAGAGTACATCGACCTTTCTTATATGGGGTTACAAAACT24850.10556710350631471No Hit
AGCGGTCAGAATTGTGCTCGCGCCTGTTTCTTATATGGGGCTTCCAGAAG24210.10284827267154442No Hit
GTTCTCGCAATGGAGCTTTTGCTGCCTTTCTTATATGGGGGCATACAGTT23590.10021440530036071No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC6850.0142.984223
CGGACGT19950.0142.860671
TCGACTG7800.0142.121639
CGGCTAG13150.0141.518741
TCGAAGT23300.0141.054582
CTCGCAT23400.0140.88319
TCGAAAG10200.0140.834782
AACGCAC19700.0140.494879
GCGCAAC15400.0140.348341
CGTAACA12400.0139.836693
CTCGAAA10500.0138.857641
CCACACG21550.0138.36922
ACTTACG11700.0138.281452
TCGACGG8700.0138.234128
GACGTAG13050.0137.875173
CGAAGTC23850.0137.801743
GACCAAT24150.0137.735121
GATCGAT8950.0137.528351
CTCTACG17850.0137.503111
TAGAAGG23700.0137.253817
Produced by FastQC (version 0.11.5)