FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5356927
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGAA22000.0115.261958
TCTAACG19350.0113.085559
CTAATGG26800.0113.028391
ATCCGAA24650.0112.497861
TACGGGC22650.0110.4790341
ATTGGAC32600.0110.2010961
ACGCGTG6450.0109.098922
CGTTAGA14250.0108.3401951
CCTTACG23100.0108.01011
ATGTCGA21450.0107.418969
TACGGTA12650.0107.004791
CGTTACG13750.0105.919159
CATCGAA17500.0105.780221
CTCTACG20850.0104.926971
GCGAGAA19450.0103.8272861
CTCGTAC18550.0103.737031
TACACCC25200.0103.6359562
CCGGTAG17600.0103.516281
GATGCGA12500.0103.178959
GCATGCG22000.0102.7679751
Produced by FastQC (version 0.11.5)