FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9303924
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGAA24550.0119.220358
CTAACGG32250.0118.8445369
CGGACGT36500.0116.9517751
TCGTACC80550.0115.7161561
GGCTCGA41400.0114.605591
CTACCCA44900.0114.151821
TCTAACG33750.0113.983338
CCTTACG36050.0112.927751
GTACGTA22850.0112.794421
GTCGACT27600.0112.08298
GCGCGAT37350.0111.153621
TAGCCGG34450.0110.521121
AGGTCCG50600.0109.830361
TCGACTA21050.0109.797339
TACCTTA39750.0109.2317051
CTAAAGC66600.0108.614662
GACAACG24050.0108.30462
TGACTAG32900.0107.7160951
TAGACCA53500.0107.709031
TCGAGCC25600.0107.255578
Produced by FastQC (version 0.11.5)