FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7449755
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG227640.30556709583066827No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA88800.11919855082482578No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG25950.0111.107498
GATATAG234700.0109.33284145
CGTTAAG28700.0109.043427
TTACGCG14600.0108.175889
GATTACG21000.0104.657252
GTTAACG21150.0103.105769
TATTACC62400.0101.729631
TCGTACC26250.0100.9699551
GCGTAAT17650.099.333899
TACGTTA21600.098.267875
TGACTAG45200.097.838871
TCGCGAG33100.097.5491641
CTCTACG36650.097.489711
TACTCGC34250.097.480381
TCGACTA14450.096.764549
CGACGGA29850.096.597429
CCTTACG20250.096.538191
TGCGAAA24050.093.986699
TCATACG19800.092.200448
CTACCCA61350.091.29771
Produced by FastQC (version 0.11.5)