FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14108151
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG182910.1296484564136009No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGA64400.0120.222271
CGTTGGG77150.0119.424131
TATAATG84750.0118.0987249
GATCGCG80000.0116.717391
GCTAATC59450.0115.8169863
AGACGTA50300.0115.557379
GCGATGA87350.0115.330239
CGTTAGT67400.0115.2730264
ACGTTAG48750.0114.922583
AACGTTG68950.0114.71841
GATATAG374800.0114.547134145
TCGAAAT35900.0114.125012
TACTCGC65850.0113.5931551
TACACGA31050.0113.532781
GTCCGAA57300.0112.9504858
CGGACTG67850.0112.606421
CGACATA41100.0112.504965
TCGCATG53250.0112.4221048
TTCGGTA42750.0111.553344
GTTAAGA68600.0110.6149448
Produced by FastQC (version 0.11.5)