FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11206790
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG301840.2693367146167636No Hit
CAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGC257120.22943233521820253No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC226050.20170807162443483No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA169110.15089958855301117No Hit
GGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTC143500.1280473712811608No Hit
CAGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA126590.11295830474203586No Hit
GTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGA121870.10874657239048825No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCC197450.0139.991841
CCGCGTA98850.0139.815964
AATCGGC174300.0138.731386
TACTTAC321000.0138.050481
TAACGCG101350.0136.847849
GATCGCC169850.0136.393286
GTAACGC102450.0135.589518
GTAAGAC122800.0135.543445
CGTAAGA96600.0135.249157
CTAATCG179600.0135.213274
GCGCAGT150350.0134.983641
TGTCGGC150300.0134.80137
CGTGCCA237900.0134.78293
ATCGGCA180850.0134.74867
TAGGACG75800.0134.601212
TCACCCG151050.0134.515768
TAGCCGA80900.0134.356497
ACGGATC174150.0134.323323
ACGTGCC246700.0134.211852
CGGATCG175100.0133.967254
Produced by FastQC (version 0.11.5)