FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7002642
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG1167861.6677419751002551No Hit
CAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGC427720.6107980387973568No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA258670.3693891534080994No Hit
GGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTC223010.3184655163008476No Hit
CAGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA162800.23248368258722923No Hit
AAACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC142500.2034946238862418No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC141720.20238075857654866No Hit
GTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGA128920.18410194323799503No Hit
TTGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACC94770.1353346351277132No Hit
TTTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGA92250.13173599335793548No Hit
TGCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTG90910.12982242987718065No Hit
AACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAG86220.12312495769453871No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA78050.11145793259172752No Hit
CATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATA77640.11087243928791447No Hit
ATGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTG70110.10011935495203095No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGAAT78850.0133.554212
ATCGATC78500.0133.490777
GCGAGAA104850.0131.643921
TCGATCC81850.0130.948978
AATCGAT80150.0129.476846
TCGCGTC66450.0128.654073
ATCGCGT68350.0127.52532
AGAATCG81050.0127.483094
GATCGCG72800.0127.2327651
CTATCGA23150.0125.529565
GTGCGCA37500.0124.63153
CGCATTA32100.0124.168176
GATATAG1015200.0122.61268145
TACACGA35400.0121.4747851
TGTGCGC39300.0119.8516542
ACGATAC28250.0119.766791
GTGTGCG49850.0119.250131
CGAGTTA32700.0118.5655755
CTAAGCG25350.0116.350715
TCTAGAC40300.0115.806635
Produced by FastQC (version 0.11.5)