FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18603212
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG316240.16999214974274335No Hit
CAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGC297030.1596659759615705No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA228510.12283362679520074No Hit
GTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGA209260.11248595135076674No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA201760.10845438948929895No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAG938900.0120.1538145
GATCGTA77550.0110.709781
CTAGCGA69750.0108.038446
GTCCCGA155850.0106.0492558
TACTTAC171600.0105.755891
ACCGAAT69850.0105.3941359
TAACACG83600.0104.7007455
TCCGTAT83100.0103.761235
TCGTAAG67300.0103.381933
GTCCGTA91300.0100.9995654
TCGTCCC155250.0100.858466
CACCGAA83350.0100.581238
AACACGA95850.0100.01316
GTATCAC104400.099.456898
CGACCTT121400.098.697381
GTGCATA130100.097.307211
CGTTGGG106100.096.924621
CACATAG181300.096.07771
TAGCAGT160000.095.871615
CGTATCA90750.095.493487
Produced by FastQC (version 0.11.5)