FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16075105
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG395280.2458957499811043No Hit
CAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGC243990.15178127918915615No Hit
TGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC205630.1279182935352522No Hit
TTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGC197020.12256218544140146No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA183140.11392771617976989No Hit
CAGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA164600.1023943545003283No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA161850.10068363472586961No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAG890950.0116.892654145
CGATTGA49550.0109.415331
TACACGA47100.0104.417191
ACGCCAG129400.0100.317031
CACTACG55800.098.649742
TACGGGC101650.097.912921
ATCGAGT72400.097.357981
CTAACGG34950.093.926859
TAGTACG51500.093.854987
CTTACCG68950.093.5515441
GCGCGAT50350.092.7499161
GCGATCA68850.092.2893453
GACGTTA79950.091.358431
TAGTTGG163150.091.014451
CGTCTAC55050.090.133161
ACTACGT61000.089.404113
GTCCGAA60300.088.810828
CAACGCT74200.087.897679
TCGCATG53900.086.449198
AACGTAG39400.085.704613
Produced by FastQC (version 0.11.5)