FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13080780
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG154910.11842565963191796No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATCGA64050.0123.757045
GATTACG24450.0118.986322
CTACCCA100700.0118.801411
TAACGCG37600.0117.934349
GCGCCAA41950.0116.814461
CGCTATC49200.0116.472431
TACGGTA44050.0114.88211
AATCCGA39300.0114.123439
ACATACG105750.0113.954411
GATATAG373100.0113.3996145
GGTATTG91200.0112.893271
GCATGCG48500.0112.298351
CCGTTCA85950.0111.147951
GAGTACG70050.0110.053857
ATCGAAC69950.0109.693277
GTGATAA71700.0109.3401958
TAGACCA90300.0109.09971
TCCACGA64200.0108.9090658
CTAACCG37700.0108.780699
CCCAACG57250.0108.2099849
Produced by FastQC (version 0.11.5)