FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19475733
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA345380.17733863983450585No Hit
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG256930.13192314764224794No Hit
ACCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACT196790.101043693708473No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCAAC145700.0140.543961
TACGGTG281050.0139.196874
CGTTAGA146450.0138.823671
TTGAACG359200.0138.817921
ACGTTAA153100.0138.466746
CAGTCGA127250.0138.127247
CGCGATA70700.0138.082062
ACGTCTA206600.0137.853535
GTACGAC139600.0137.74028
TATGCCC149650.0136.981191
TACTCGC167450.0136.509489
CGTCTAC210750.0135.036216
ATCGCGC120600.0134.930087
CGCAACG147800.0134.75082
CGGACTG231350.0134.588931
CAACACG164100.0134.43969
CGAAGTC121750.0134.174363
TGTACGA143200.0134.075427
GAAACGA232050.0133.756979
CCTTACG294750.0133.60251
Produced by FastQC (version 0.11.5)