FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004852532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9836686
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG516140.5247092364237306No Hit
CAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGC425130.43218823900651093No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA307690.31279843638396104No Hit
GGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTC272840.277369837768533No Hit
AAACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC231400.23524182839627086No Hit
GTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGA206130.20955228214055016No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC204530.20792571807212307No Hit
CAGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA179120.18209384746041501No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA148120.15057916863463974No Hit
GACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTT117600.11955245902939263No Hit
CCACCTACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG116920.11886116930031111No Hit
TTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGC110760.11259889763686672No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)