FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9156654
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG191400.2090283197333873No Hit
CAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGC104750.11439768282169448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAA27250.0123.0477758
CGTTAGA46200.0114.236461
GATATAG372100.0111.82088145
CGTACGA20900.0110.882497
ACGATAC26900.0107.1646651
CGGACTG38050.0106.7551
CCGGTAG47200.0106.726631
CATCGAA38500.0104.750891
TACGAAA32300.0104.258089
ACGTCAA39350.0103.968151
TACGGGC35100.0103.905681
ACTAACG16650.0103.6088642
CTCGAAA44250.0102.1614461
TAACGCG16400.0102.09452
AACAGTA65000.0100.719696
CCTTACG27450.0100.509141
TCCCGAT35350.099.6700441
GACACGC31950.099.5678941
CAGTCGA24650.096.364318
TCGCTGA47450.096.000568
Produced by FastQC (version 0.11.5)