FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004852536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13239208
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG823420.6219556335998346No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC598420.45200589038256667No Hit
CAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGC449010.33915170756438No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA350140.26447201373375206No Hit
CAGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA271270.20489896374465905No Hit
GTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGA267990.20242147415464729No Hit
GGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTC256540.19377292055536857No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA234150.17686103277477022No Hit
AAACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC207940.15706377602043867No Hit
GCTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCT185860.1403860412193841No Hit
GACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTT173180.13080842902385098No Hit
TGCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTG154620.11678946353890657No Hit
GCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATA152440.11514283936017926No Hit
CAGTCCTTTGAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCT143650.10850346939182466No Hit
ATGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTG142280.1074686642886795No Hit
AACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAG141160.10662269223355354No Hit
CCCTCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGT140040.1057767201784276No Hit
TTCTCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGT136660.10322369736920818No Hit
AGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTA136130.10282337130740751No Hit
CCACCTACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG135580.1024079386017653No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)