FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9945922
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG335220.33704265929292426No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC270770.2722422315397205No Hit
CAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGC193680.194733077536703No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA189660.19069121997940464No Hit
GTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGA153920.15475689433317494No Hit
CAGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA144540.14532589336614543No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA134710.1354424456576273No Hit
GGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTC131360.13207423102654534No Hit
AAACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC126910.12760003547182452No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAG1138100.0119.45413145
TACACGA35200.0111.4319841
GTAAAAT277250.0108.80799145
TACGGAT48200.0107.044151
CTCCTTA408550.0106.76106145
CGTCTAC41250.0104.438711
TACTTGT96300.0100.883181
AAACGCG37650.099.0655069
CCAAACG35800.098.318097
AGCATCA318200.095.829506145
GTACGTA41850.095.8121
CGTTGGG48800.095.4385761
AATCATG383400.094.81617145
TTTGCGC45350.094.515231
AACACGT55700.094.198471
CTAGCGA13200.093.821516
CACCGTA32500.092.925079
GCGTAAT21500.092.6348659
ATCCGAA25150.091.145741
ATTCTAC81250.090.281951
Produced by FastQC (version 0.11.5)