FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11602654
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACGA41200.0112.969571
CGTCTAC66950.0111.405641
CGAACAT42800.0111.299141
GACGCGT78400.0110.5576251
CGGCTAG67900.0108.237861
CTACGTC60950.0105.761441
CGTTAGA53400.0105.028531
TAAACCG49250.0104.8570861
GATTACG26600.0103.673852
GATATAG259550.0102.04971145
TCGCTAG30650.0101.084838
AGCGTGA42300.0100.861528
CGTGCAA32650.0100.673867
CTTACCG47600.0100.07571
CGATTGA21950.099.882591
TTTGCGC86100.099.063021
GCATGCG45800.098.124511
CTAACGG27800.097.7428749
ACGCGTA27250.097.738772
GTACGAC18000.097.418529
Produced by FastQC (version 0.11.5)