FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5221689
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA225740.4323122269441937No Hit
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG135950.26035637128139955No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT129990.24894243988870268No Hit
GGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTC101450.19428579526662731No Hit
CAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGC101330.1940559845674455No Hit
ACCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACT71340.1366224606635899No Hit
GCGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTC68900.13194964311355964No Hit
CAGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA68250.1307048351596581No Hit
ACACCAAAGCCCTAATACTAAGCGGCTTTCTTATATGGGGGCATTTTGGC63570.12174221789156728No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC56950.1090643276533704No Hit
CAACTAGGTGATGTCTCCCGTGTTGGTTTCTTATATGGGGACTCCTGGTT54920.105176696658878No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG47500.0129.770372
CGTTGGG51400.0126.872631
AGATCGA51650.0124.7559368
CGTACGA10050.0123.327477
TACGTGA34350.0122.807825
ATTACGT51150.0120.223113
ACTAGGT35150.0120.0702443
ACGTGAC35050.0119.5279856
TAGGCAT61050.0119.4631
CGTGACT35800.0118.441157
GATATAG538550.0117.64835145
GATCGAT55300.0116.652669
TATCCGA22100.0115.774316
CGACCTT43100.0115.483211
GTACGTA20800.0115.2289661
TACACGA18450.0114.763431
CATAAGA50300.0114.703028
GTATGAC34850.0114.390429
ATAAGAC50450.0114.361989
ACGGATA18700.0112.792739
Produced by FastQC (version 0.11.5)