FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13611725
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG179700.13201853549054215No Hit
CAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGC159170.11693595043978629No Hit
ACCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACT149210.10961872944097828No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGC89500.0124.00021
GATATAG650000.0116.84443145
TACTTAC198750.0112.787551
CGATTGA36900.0105.365331
CCTATCG41000.0101.515814
AGCCTAA134000.0100.537041
GCTATGC76250.098.578789
CCGTACA45100.095.788699
CCTTACG51800.095.205321
CGACCTT105200.093.4080051
CTTACTC178650.093.083373
CGTTAGA31500.091.6960141
CCTACAC115600.090.790171
GTTACGT38850.090.488888
ACCGTAC38450.088.602515
ATTGGTG202600.088.442851
CGGACTG58400.088.347191
CCGAATG33400.088.326799
AATCCGA43750.088.141029
GTCGAAC28150.088.0627448
Produced by FastQC (version 0.11.5)