FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10576942
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA150800.1425742903761787No Hit
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG144710.13681648249560222No Hit
CCACCTACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG143690.13585212058456972No Hit
TGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC133030.12577359316142606No Hit
TTGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT113710.10750744402304561No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAG598200.0116.66492145
GTCGAAC31600.0108.958018
CGCTATC44900.0107.242121
GTCGACT31300.0104.6758968
GAACCTA63250.0103.907621
ACGATGT71700.0102.5300751
TCGACTA26150.0101.452339
CCACCTA113450.099.854711
ACTAACG34350.099.227292
ACGATAC36950.098.681931
TACGGGC34700.095.54711
TAGTACG36100.095.3759847
TGTCGAA50650.094.882627
ATGCGAT36250.094.503591
AACGTTG49850.094.381151
CGTATGG39050.094.1108257
ATTATCG31550.092.403142
GCGCAAC31850.090.709661
TAAACCG40950.090.658361
GACGCTT77300.090.3961949
Produced by FastQC (version 0.11.5)