FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17354529
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAG436000.0108.86603145
GTACGAT72950.0107.023559
TAGTACG81450.098.702787
ATTATCG49750.091.4432142
AGTACGA87050.091.020748
CCTTACG59800.090.1124341
TCGATAG56700.088.8568048
ACATACG83750.088.46071
CAATCGA48650.085.678918
CGTATGA41100.081.8399057
CGTTAGA54900.081.0212551
GTTAACG42550.079.902849
CGTTTCG37250.079.595047
CGTCTAG46050.078.709897
GTCGACT47850.078.324498
CGCTATC54200.077.8971
ACCGTAA86400.076.632611
GTAATAG133100.076.413238
CTGATAG121800.075.912531
TACGGTC56300.075.8920444
Produced by FastQC (version 0.11.5)